Composite

Part:BBa_K4807026:Design

Designed by: Vaios Styliaras, Efi Chatzopoulou   Group: iGEM23_IOANNINA   (2023-10-11)


hemA(s) insert


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 236
    Illegal PstI site found at 993
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 236
    Illegal PstI site found at 993
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 236
    Illegal PstI site found at 993
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 236
    Illegal PstI site found at 993
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1070
    Illegal SapI.rc site found at 221


Design Notes

Minor base modifications have been made for proper Primer Design, for keeping translational frame intact etc


Source

E.coli genome.

References